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The relationship between the electrical and chemical components of Δ\Delta p is given by the equation:


A) Δ\Delta p = Δ\DeltaΨ\varPsi - (2.3RT/F) Δ\Delta pH
B) Δ\Delta p = Δ\DeltaΨ\varPsi - 60 Δ\Delta pH
C) Δ\Delta pH = |pHo - pHi|
D) both A and B
E) none of the above

F) A) and D)
G) A) and C)

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The F1Fo ATP synthase is a remarkably complex enzyme, its subunit structure highly conserved across life domains. Is it possible to use this enzyme as an antibiotic target?

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Differences among prokaryotic F1Fo ATPases...

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In seawater, sulfate is the most common anion after chloride. Which of the following is correct with respect to the use of sulfate by some bacterial groups?


A) Oxidized sulfur-containing molecules have redox potentials lower than those of the nitrogen series.
B) Oxidized sulfur molecules such as sulfate and sulfite serve as electron acceptors.
C) Sulfate and sulfite can receive electrons from hydrocarbons.
D) Sulfate-reducing archaea and bacteria are widespread in the ocean.
E) All of the above.

F) A) and E)
G) B) and D)

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E

Ferroplasma acidarmanus produces large amounts of sulfuric acid through:


A) oxidation of Cu+ with Cu(NO3) 2
B) oxidation of FeS2 with Fe3+
C) oxidation of Fe3O2 with Fe2+
D) all of the above
E) none of the above

F) C) and E)
G) D) and E)

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What is the direction of the proton current in the chloroplast F1Fo ATP synthase during ATP synthesis?


A) from stroma to cytoplasm
B) from cytoplasm to stroma
C) from lumen to stroma
D) from stroma to lumen
E) none of the above

F) B) and C)
G) A) and B)

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Which of the following is NOT correct about the process of methanogenesis?


A) CO2 and H2O are weak electron acceptors, whereas CH4 and H2 are strong electron donors.
B) CH4 synthesis from CO2 releases energy for growth.
C) CH4 and water can be formed from CO2 and 4H2 from a hydrogen donor.
D) Oxygen atoms in CO2 are reduced one at a time.
E) Oxygen atoms in CO2 are reduced to water at the same time.

F) C) and D)
G) B) and E)

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The chemiosmotic theory states that the proton potential is composed of:


A) [ATP] and [ADP]+[Pi]
B) a pH gradient and Na+ gradient
C) pH and pNa+
D) Δ\Delta pH and Δ\DeltaΨ\varPsi
E) none of the above

F) A) and E)
G) C) and D)

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In anaerobic soils, some yeasts and filamentous fungi can reduce nitrate to nitrite and nitrite to:


A) ammonia (NH3)
B) nitric oxide (NO)
C) urea
D) all of the above
E) none of the above

F) B) and E)
G) A) and C)

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What are methanotrophs? What is their role in the global carbon cycle?

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Methanotrophs are organisms that oxidize...

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What is the chromophore group in bacteriorhodopsin?


A) tocopherol
B) β\beta -carotene
C) folic acid
D) retinal
E) none of the above

F) A) and D)
G) B) and C)

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D

What is dissimilatory metal reduction? How is it different from assimilatory metal reduction?

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Dissimilatory metal reduction is an impo...

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Which of the following enzymes is embedded in the membrane as an ETS component?


A) pyruvate dehydrogenase
B) 2-oxoglutarate dehydrogenase
C) succinate dehydrogenase
D) aconitase
E) none of the above

F) A) and B)
G) A) and C)

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What is lithotrophy?


A) breakdown of molecules using light energy
B) oxidation of organic electron donors to CO2 and H2O
C) photolysis of H2S or H2O coupled to CO2 fixation
D) oxidation of inorganic electron donors such as Fe2+ using O2 or anaerobic electronic acceptors
E) none of the above

F) B) and E)
G) A) and E)

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Neisseria species are known to use dissimilatory reduction of nitrate to ammonium. How can this reaction be used to distinguish Neisseria gonorrhoeae from other related species?


A) N. gonorrhoeae will test negative for terminal NO reductase.
B) N. gonorrhoeae will test positive for terminal NO reductase.
C) All Neisseria species test negative for terminal NO reductase.
D) None of the enzymes involved in dissimilatory reduction of nitrate are known from Neisseria.
E) none of the above.

F) B) and C)
G) A) and B)

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What are nitrifiers? What damages and benefits may they have for human activities?

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Reduced nitrogen forms, such as fertilizer-derived NH3, support the growth of bacteria called nitrifiers. Besides ammonia, these microbes can sequentially oxidize nitrogen compounds such as hydroxylamine to nitrous and nitric acids. Acidification of the overfertilized soils, plus a decrease in reduced nitrogen (assimilable by plants), leads to degradation of environmental quality. However, nitrifiers can be used to eliminate NH3 from sewage in treatment plants.

In the Rhodospirillum rubrum photosystem II, bacteriochloropyll P870 can absorb weak infrared wavelengths in the __________ range.


A) 65-700
B) 750-850
C) 800-1,100
D) all of the above
E) none of the above

F) None of the above
G) A) and C)

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Which of the following is correct with respect to anaerobic photosystem I?


A) It can be found in chlorobia (the green sulfur bacteria) and chloroflexi (filamentous green bacteria) .
B) It separates electrons associated with hydrogens from H2S, HS-, or H2.
C) It separates electrons from Fe2+.
D) All of the above.
E) None of the above.

F) A) and B)
G) C) and D)

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The anammox reaction consists of __________ and, as a consequence, __________.


A) reduction of sulfate by ammonia; sulfur and nitrogen are incorporated into amino acids
B) reduction of ammonium to form nitrite; nitrite can be absorbed by plants
C) anaerobic ammonium oxidation by nitrite; half the biosphere's nitrogen is returned as N2 to the atmosphere
D) reduction of ammonium by oxygen; ammonium becomes ammonia
E) reduction of N2 to ammonium; nitrogen atoms are incorporated into amino acids

F) C) and E)
G) None of the above

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What are facultative phototrophs? Provide an example and its possible use in biotechnology.

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Microorganisms that use a combination of...

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What are the units of E°'?


A) kJ/mol
B) kcal/mol
C) mV
D) ohm
E) faraday

F) B) and E)
G) C) and D)

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